Notes from NEET topper

Components of Vectors:

Vectors typically consist of the following components:

  1. Origin of Replication: This is the DNA sequence where DNA replication initiates.

  2. Selectable Marker: Genes conferring resistance to antibiotics or other selective agents.

  3. Multiple Cloning Site (MCS): A region with multiple unique restriction enzyme recognition sites for DNA insertion.

  4. Promoters and Regulatory Elements: If required, these elements control gene expression.

  5. Plasmid Backbone: The basic structure of the vector that provides stability and replication machinery.